The Recognition of a vlhA Protein from the F-Strain of Mycoplasma gallisepticum with Monoclonal Antibody 6F10
نویسنده
چکیده
The goal of this project is to identify the genes encoding M. gallisepticum F-strain surface proteins recognized by specific antibody reagents to characterize the individual role of each gene product in host colonization. Here we report the characterization of a 70-kDa surface protein recognized by monoclonal antibody 6F10. The surface protein was gel isolated and digested with trypsin for evaluation with mass spectrometry. The results showed that 19 out of the 21 tryptic peptides matched with proteins of the vlhA lipoprotein family. We suggest that monoclonal antibody 6F10 is specific for the predominantly expressed vlhA protein (formerly pMGA) of the F-strain of M. gallisepticum.
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